Background: Several GC and HPLC methods have been proposed for the separation of aldose
Method: We developed a general HPLC method for the discrimination of (D,L)-monosaccharide components
from natural products. The reaction involves the preparation of L-cysteine (3-nitrophenyl)
methyl ester hydrochloride, which reacts with aldoses leading to thiazolidine derivatives.
Results: Direct HPLC analysis with reversed-phase column and UV detection, discriminated enantiomeric
D- and L-monosaccharides in a highly sensitive manner.
Conclusion: This method was applied for the determination of the absolute configurations of monosaccharides
in the natural poliumoside B, a tetraglycoside obtained from Teucrium polium.