Background: Infectious diseases caused by various pathogens including bacteria, fungi,
viruses, and parasites are a very common danger to people health.
Objective: The main aim of the present study was to evaluate the in vitro pharmacological effects of
and Atropa belladonna
extracts through antibacterial and antioxidant activities to
find basic information for the development of potential therapeutic drugs from these plants.
Methods: In the current research, the antioxidant constituents of Atropa accuminata
were assessed through free scavenging methods and thin layer chromatography (TLC) followed
by DPPH (2, 2-Diphenyl-1-picrylhydrazyl) spray technique. Antibacterial activity of the leaf and
root extracts was analysed for nine bacteria through agar well diffusion method. The effect of standard
antibiotics was also determined by the agar disc diffusion method. Total phenolic and total flavonoid
contents were estimated by folin-ciocalteau and aluminum chloride methods.
Results: Both medicinal plants exhibited low to high antibacterial activity against all tested bacterial
pathogens. TLC-spot screening indicated the presence of antimicrobial agents. Phenolic contents were
higher in ethanolic and DMSO extracts of A. belladonna
roots compared to the leaf extracts whereas
flavonoid contents were higher in the DMSO extracts of A. belladonna
leaf. High-performance liquid
chromatography indicated the presence of a variety of sugar and organic acids.
Conclusion: Such findings would be useful in promoting research aiming at the discovery of new antimicrobial
agents and antioxidants.