Introduction: Acinetobacter baumannii has emerged as an important nosocomial
pathogen in the past decades. Due to the prevalence of A. baumannii across
the world, suitable typing methods to investigate the epidemiological distribution of
the organism have been developed. The aim of this study was to investigate the epidemiological
and molecular diversity of A. baumannii strains isolated from nosocomial
infections of hospitalized children in Children Medical Center Hospital
(CMC), an Iranian referral hospital, in Tehran, Iran.
Material and Methods: A total of 27 non-duplicate clinical A. baumannii isolates
were collected during October 2013 to March 2014 and tested for antimicrobial resistance
to several antibiotic agents. The genetic similarity of the strains was investigated
by amplification of Random amplified polymorphic DNA-polymerase chain
reaction (RAPD-PCR) method.
Results: One predominant RAPD profile (type B) was identified in 15 strains (56%
of all typed isolates). Other clusters depicted in the dendrogram, namely A, C, and D
comprised 6 (22%), 5 (19%) and 1 (3%) isolates, respectively. All A. baumannii
strains were resistant to all antibiotics except colistin.
Conclusion: This study highlights the clonal spread of multidrug-resistant A. baumannii
in our hospital. Therefore, the factors responsible for dissemination of such
isolates need to be identified, controlled, and prevented to avoid major outbreaks.