System Level Meta-analysis of Microarray Datasets for Elucidation of Diabetes Mellitus Pathobiology

Author(s): Aditya Saxena*, Kumar Sachin, Ashok Kumar Bhatia.

Journal Name:Current Genomics

Volume 18 , Issue 3 , 2017

Graphical Abstract:


Abstract:

Background: Type 2 diabetes (T2D) is a common multi-factorial disease that is primarily accounted to ineffective insulin action in lowering blood glucose level and later escalates to impaired insulin secretion by pancreatic β cells. Deregulation in insulin signaling to its target organs is attributed to this disease phenotype. Various genome-wide microarray studies from multiple insulin responsive tissues have been conducted in past but due to inherent noise in microarray data and heterogeneity in disease etiology; reproduction of prioritized pathways/genes is very low across various studies.

Objective: In this study, we aim to identify consensus signaling and metabolic pathways through system level meta-analysis of multiple expression-sets to elucidate T2D pathobiology.

Method: We used ‘R’, an open source statistical environment, which is routinely used for Microarray data analysis particularly using special sets of packages available at Bioconductor. We primarily focused on gene-set analysis methods to elucidate various aspects of T2D.

Result: Literature-based evidences have shown the success of our approach in exploring various known aspects of diabetes pathophysiology.

Conclusion: Our study stressed the need to develop novel bioinformatics workflows to advance our understanding further in insulin signaling.

Keywords: Type 2 Diabetes, Insulin-signaling, Microarray, Meta-analysis, Bioconductor, Gene-set analysis.

Rights & PermissionsPrintExport

Article Details

VOLUME: 18
ISSUE: 3
Year: 2017
Page: [298 - 304]
Pages: 7
DOI: 10.2174/1389202918666170105093339
Price: $58

Article Metrics

PDF: 10
HTML: 1
EPUB: 0
PRC: 0