Background: Helicobacter pylori are gram-negative spiral shaped bacteria,
with sheathed flagella. H. pylori infection is one of the most common chronic infections
in humans. Infection is usually acquired during childhood, and becomes a
lifelong infection in most people unless treated. The aim of this study was to evaluate
serum levels of oxidative stress indices in children with H. pylori infection.
Material and Methods: The present study was carried out on 60 children infected
with H. pylori including 28 males, 32 females with their age ranging from 7-10 years
and mean age value of 8.5 ± 1.65 ( Group I). This study included also 60 children as
a control group including 26 males, 34 females with their age ranging from 7-11 and
mean age value of 8.99 ± 1.63 (Group II). For all children in groups I the following
were done: Diagnosis of H. pylori infection through H. pylori stool antigen testing
using enzyme immunoassay kit and gastric antrum mucosal biopsies which were
tested for urease activity using Campylobacter like organism test (CLO test). Measurements
of serum oxidative stress markers including Superoxide dismutase (SOD),
Malondialdhyde, Glutathione, Catalase and Nitric oxide were done in patients and
Results: Serum nitric oxide and reduced glutathione were significantly lower in patients
compared to controls while serum MDA, Serum catalase and Serum SOD were
significantly higher in patients compared to controls (nitric oxide was 91.111 ±6.366
in patients versus 107.211±2.121 in controls with p value of 0.001, reduced
glutathione in patients was 2.457± 0.081 versus 2.889±0.491 in controls with p value
of 0.001, serum MDA in patients was 140.22±5.18 versus 116.22±2.98 in controls
with p value of 0.001, catalase was 401.645± 4.344 versus 278.221±71.712 in controls
with p value of 0.001 and SOD in patients was 16.936±9.145 versus
5.578±0.231 in controls with p value of 0.001).
Conclusion: H. pylori infection is associated with oxidative stress with significantly
lower serum nitric oxide and reduced glutathione and significantly higher serum
MDA, catalase and SOD in patients compared to controls.
Recommendations: Antioxidants may be beneficial adjuvant treatment in H. pylori
infection as H. pylori infection is associated with oxidative stress.