Background: Vitamin has an important function in biochemical processes and plays an important
role in the main process of the human body. Vitamin products are used to maintain health, restore
vitality, control weight and help in disease prevention. To comply with regulatory requirements
and to establish nutrient intake in some populations, the quality of vitamins in food and nutritional supplements
needs to be ensured.
Methods: This study aims to obtain a simultaneous method for the quantification of thiamine hydrochloride,
riboflavin, nicotinamide, dexpanthenol, pyridoxine hydrochloride, cyanocobalamin and ascorbic
acid in pediatric syrup by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
(UPLC-MS/MS). ACQUITY UPLC® BEH Shield C8 column (2.1 mm x 50 mm, 1.7 m), mobile phase is
a mixture of 10 mM ammonium formate in 0.2% formic acid pH 2.8 with methanol by gradient elution
at a flow rate of 0.4 mL/min achieved in 6.5 minutes was used for separation. Ionization method was
positive electrospray ionization (ESI +) with Multiple Reaction Monitoring (MRM).
Result: Confirmation result showed 7 water-soluble vitamin parent ions and daughter ion of thiamine
HCl at m/z 265.15> 122; riboflavin at m/z 377.20> 243.10; nicotinamide at m/z 123> 80.10; pyridoxine
HCl at m/z 170.10> 152.00; cyanocobalamin at m/z 678.45> 147.05; dexpanthenol at m/z 206.05>
76.00 and ascorbic acid at m/z 177.00> 94.90. The results of method validation showed that the method
fulfills the acceptance criteria of precision, recovery and linearity
Conclusion: The method can be used for the routine analysis of determination of seven water soluble
vitamins in pediatric syrup.