Background: The erythrocytes are primarily exposed to xenobiotics and are
highly susceptible to endogenous oxidative damage due to presence of high concentrations
of polyunsaturated fatty acids (PUFAs).
Objective: The aim of the present investigation is to assess the protective ability of Kalanchoe
blossfeldiana extract (MEKB) against chemical oxidative stress induced by 2,2`-
azobis(2-amidinopropane) dihydrochloride (AAPH) on rat erythrocyte.
Method: The inhibition rates of haemolysis, concentration of malondialdehyde and reduced
glutathione were evaluated as the biomerkers of oxidative status.
Results: Phytochemical analysis indicates the presence of phenolic compound (249.1±9.4 mg of
gallic acid equivalent per gm of dry extract) and flavonoids (335±3.7 mg quercetin equivalent per
gm of dry extract) in substantial amounts. In addition, the major phenolic compound determined
by HPLC analysis was quercetin. MEKB displayed significant antioxidant effect by ferric reducing
ability and also oxygen and DPPH radical scavenging activity. MEKB significantly protected
erythrocyte against AAPH induced haemolysis and this was further substantiated by its ability to
reduce membrane lipid peroxidation along with restoration of glutathione.
Conclusion: The present findings suggest that MEKB may have the potential to act as natural
antioxidant, thereby providing membrane stability to erythrocytes and protecting the cells
from oxidative damage.