Background: Propolis has been used as a natural health product mainly
due to the presence of polyphenols, flavonoids, phenolic aldehydes, amino acids,
vitamins and others bioactive constituents. To this natural substance are attributed
different biological and pharmacological properties which are influenced by its
chemical composition and organoleptic properties. The aim of this work was to
evaluate the physicochemical properties and parameters of green propolis collected
during a period of six years (2008-2013) in the state of Minas Gerais, located at the
southeastern region of Brazil.
Methods: The methodology were in accordance with Brazilian legislation on the
identity and quality standards of propolis. The evaluated parameters of
hydroalcoholic from green propolis were total flavonoids, antioxidant activity -
DPPH method, oxidation index, wax content, humidity and insoluble impurities.
Results: Propolis samples collected in different seasons during the years 2008 to
2013 presented mean values of total flavonoids (3.4 ± 0.11 mg/g), antioxidant
activity DPPH (4.76 ± 0.16 μg/mL), oxidation index (3, 4 ± 0.33 seconds) and wax
(15.14 ± 0.78% m/m), which are in accordance with Brazilian legislation.
Conclusion: Green propolis did not show abrupt seasonal changes during the six
years of investigation, and may be considered as an adequate functional ingredient.