Troubles and Efficiency of aDNA
Pp. 160-182 (23)
Marc Simon and Assumpcio Malgosa
From the establishment of a first set of authenticity criteria to their
progressive improvement in parallel with this field’s technology advances, the fight to
overcome contamination has not ceased over the years. On its part, another problem at
the time of recovering ancient genetic material can be caused by properties which may
be inherent to the samples, as well as by their interaction with the elements where they
are located. A summary from the evolution in these factors’ knowledge and the
solutions that scientists have given them before the arrival of next-generation
sequencing techniques is provided. Finally, a thorough description of the tissues from
which ancient genetic material is recovered and the developments to do so from
different source organisms is provided.
Ancient tissue, Authenticity criteria, Bone, DNA extraction,
Feasibility, Hydroxyapatite, Inhibition, Phenol-chloroform, Silica, Sterility, Teeth.
Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain.