Anthropology: Current and Future Developments

Volume: 2

Genomics in Biological Anthropology: New Challenges, New Opportunities

Molecular methodologies are crucial to our understanding of human population diversity, as well as our evolutionary relationships with nonhuman primates. The completion of the Human Genome Project ...
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Troubles and Efficiency of aDNA

Pp. 160-182 (23)

Marc Simon and Assumpcio Malgosa

Abstract

From the establishment of a first set of authenticity criteria to their progressive improvement in parallel with this field’s technology advances, the fight to overcome contamination has not ceased over the years. On its part, another problem at the time of recovering ancient genetic material can be caused by properties which may be inherent to the samples, as well as by their interaction with the elements where they are located. A summary from the evolution in these factors’ knowledge and the solutions that scientists have given them before the arrival of next-generation sequencing techniques is provided. Finally, a thorough description of the tissues from which ancient genetic material is recovered and the developments to do so from different source organisms is provided.

Keywords:

Ancient tissue, Authenticity criteria, Bone, DNA extraction, Feasibility, Hydroxyapatite, Inhibition, Phenol-chloroform, Silica, Sterility, Teeth.

Affiliation:

Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain.