Background: Peripheral blood stem cells (PBSCs) are now established curative treatment for
patients with various hematologic malignancies and are proposed to be superior to other cell sources for
cell based therapy.
Aims: The aims are to characterize the PBSCs by constructing proteome using 2D-gel electrophoresis
and analyze the kinetic parameters of pirarubicin transport of PBSCs compared with peripheral blood
mononuclear cells (PBMCs), and erythromyelogenous leukemic (K562 and K562/adr) cells.
Methods: Whole cell proteomes of PBMCs, PBSCs and erythromyelogenous leukemic cells were
separated by 2D-gel electrophoresis and analyzed by multivariate and principle component analysis.
The parallel series of experiments were measured by pirarubicin transport parameters by the cells using
Results: The PBSCs were initially found in 1% in the fraction of PBMCs and majority of them were
found in the culture after 14 days. Multivariate and principle component analysis revealed the proteins
that might be considered as biomarkers of PBSCs (4 proteins), PBMCs (9 proteins), K562 (4 proteins)
and K562/adr (9 proteins) cells. The analysis of transport of pirarubicin by these cell models indicated
that PBSCs and PBMCs possessed exocytotic pathway by which the mean rate constant was equal to
0.056 + 0.001 and 0.56 + 0.01 pL.cell-1.sec-1, respectively. While the two cancer lines showed the lack
of the exocytosis activity.
Conclusion: The overall results suggested that both whole cell proteome and kinetic parameter of exocytosis
should be considered as characteristics of PBSCs, PBMCs, K562 and K562/adr cells.