The Biology of β-D-mannuronic acid (M2000) on Human Dendritic Cell Based on MicroRNA-155 and MicroRNA-221

Title:The Biology of β-D-mannuronic acid (M2000) on Human Dendritic Cell Based on MicroRNA-155 and MicroRNA-221

Volume: 14 Issue: 1

Author(s): Nakisa Tabrizian, Nazanin Arjomand Fard, Ali Farazmand, Rahil Eftekhari, Farzaneh Tofighi Zavareh and Abbas Mirshafiey

Affiliation:

Keywords: M2000, β-D-mannuronic acid, miRNA-155, miRNA-221, dendritic cells, DCs.

Abstract: Background: The aim of this study was to evaluate the effect of ß-Dmannuronic acid (M2000) on related miRNAs to dendritic cells (DCs) differentiation. DC-based immunosuppressive drugs can suppress the progression of autoimmune diseases, however, their notable side effects in increasing the risk of infectious diseases and cancers should be considered. The β-D-mannuronic acid, as a novel non-steroidal anti-inflammatory agent, has been tested in various experimental models.

Method: The effect of M2000 on expression of miRNA-155 and miRNA-221 was examined. To investigate how M2000 affects differentiation of human dendritic DCs in a defined inflammatory environment, human peripheral blood mononuclear cells were isolated from healthy blood and the monocytes were purified using anti-CD14 microbeads. The so isolated monocytes were subsequently incubated in the presence of M2000 in two different doses (3 and 6 mMol/well) adding granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 for inducing monocytes to immature DC and lipopolysaccharide for running DC differentiation. The expression of miRNA-155 and miRNA-221 were examined with Real Time PCR.

Results: The results demonstrate that M2000 has no significant side effect on expression of miR-155 and miR-221 in both immature DC and mature DC process in vitro.

Conclusion: Our findings show that β-D-mannuronic acid is a safe agent which has no adverse effect on regulatory miRNA-155 and miRNA-221 in dendritic cells.

Cite this article as:

Tabrizian Nakisa, Fard Arjomand Nazanin, Farazmand Ali, Eftekhari Rahil, Zavareh Tofighi Farzaneh and Mirshafiey Abbas, The Biology of β-D-mannuronic acid (M2000) on Human Dendritic Cell Based on MicroRNA-155 and MicroRNA-221, Current Drug Discovery Technologies 2017; 14 (1) . https://dx.doi.org/10.2174/1570163813666161123121952

DOI https://dx.doi.org/10.2174/1570163813666161123121952	Print ISSN 1570-1638
Publisher Name Bentham Science Publisher	Online ISSN 1875-6220