Single-Cell Sequencing for Drug Discovery and Drug Development

Author(s): Hongjin Wu, Charles Wang*, Shixiu Wu*.

Journal Name: Current Topics in Medicinal Chemistry

Volume 17 , Issue 15 , 2017

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Abstract:

Next-generation sequencing (NGS), particularly single-cell sequencing, has revolutionized the scale and scope of genomic and biomedical research. Recent technological advances in NGS and singlecell studies have made the deep whole-genome (DNA-seq), whole-epigenome and whole-transcriptome sequencing (RNA-seq) at single-cell level feasible. NGS at the single-cell level expands our view of genome, epigenome and transcriptome and allows the genome, epigenome and transcriptome of any organism to be explored without a priori assumptions and with unprecedented throughput. And it does so with single-nucleotide resolution. NGS is also a very powerful tool for drug discovery and drug development. In this review, we describe the current state of single-cell sequencing techniques, which can provide a new, more powerful and precise approach for analyzing effects of drugs on treated cells and tissues. Our review discusses single-cell whole genome/exome sequencing (scWGS/scWES), single-cell transcriptome sequencing (scRNA-seq), single-cell bisulfite sequencing (scBS), and multiple omics of single-cell sequencing. We also highlight the advantages and challenges of each of these approaches. Finally, we describe, elaborate and speculate the potential applications of single-cell sequencing for drug discovery and drug development.

Keywords: NGS, Single-cell sequencing, RNA-seq, Drug discovery, Single-cell bisulfite sequencing.

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Article Details

VOLUME: 17
ISSUE: 15
Year: 2017
Page: [1769 - 1777]
Pages: 9
DOI: 10.2174/1568026617666161116145358
Price: $58

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