Background: Glycoalkaloids production from natural plants suffers from many problems,
including instability and scarcity. The scope of the study was to biosynthesize these important
metabolites via plant tissue culture as alternative way besides, evaluating their biological activities.
Method: Solasodine glycoalkaloids production potentiated in cultures of Solanum laciniatum
on Murashige and Skoog (MS) media containing growth hormones and phenylalanine. The biosynthesized
glycoalkaloids were examined for their biological activities.
Results: Extracted glycoalkaloids from calli and regenerated shootlets were found to be much
higher than intact plant. HPTLC and HPLC examination illustrated that glycoalkaloids were solasonine
and solamargine in addition to solasodine aglycone. Electron Spin Resonance technique
(ESR) was used for measurement of the antioxidant capacity of the separated glycoalkaloids extract
(83.87%) against DPPH•. In vitro
glycoalkaloids possessed cytotoxic activity against human carcinoma
cell lines of lung, liver, brain, lymphoblastic leukemia and breast. IC50
values (μg/mL) were
0.84, 11.79, 12.58, 14.88 and 23.40, respectively. Virucidal activity of the glycoalkaloids against
performed using plaque reduction method and exhibited marked activity (88.1%).
Glycoalkaloids also have pronounced anti-inflammatory effect.
Conclusion: The obtained results explore in vitro
solasodine glycoalkaloids biosynthesis that could
incorporate in pharmaceuticals according to the examined biological efficiency.