Background: The natural products have increasing important for the development of anticancer
agents. Colchicum baytopiorum C.D. Brickell (C. baytopiorum), an endemic species for Turkey,
contains colchicine and its derivatives. Stimulation of apoptotic and autophagy-mediated cell deaths are
effective strategy for anticancer therapies.
Aim: The aim of the study is to determine the role of the extract on both apoptotic and autophagic cell
death in HeLa cell line.
Methods: The cell viability of C. baytopiorum (0.1 mg/ml) was determined by MTT assay. Active
caspase-3 and t-Bid expressions were evaluated by immunohistochemical method. The mRNA expression
of apoptotic regulatory genes (Bcl-xL, Bid, Bad, PUMA, NOXA, Caspase-3, -8, -9, Fas, FADD,
TRADD, TRAF2, TNF, TNFR1), autophagic cell death related genes (Atg5-12, Beclin-1, DAPK), and
also both autophagic and apoptotic cell death regulatory genes (Bif-1 and BNIP-3) were investigated by
Results: We determined that the expressions of both apoptotic and autophagic regulatory genes were
significantly increased in the treatment group compared to control group. Also, we showed that C. baytopiorum
crude extract induces the cross-connection between apoptotic and autophagic cell deaths in
Conclusion: We suggested that this endemic plant extract seems to be a new promising therapeutic
approach in cancer.