Background: Active pharmaceutical ingredients have been reported to vary with
environmental and edaphic conditions from region to region. Global resurgence in traditional
and alternative health care systems has necessitated the quality standardization and authentication
of medicinal plants with respect to their active ingredients which have also been
stressed by World Health Organization and modern pharmacopoeias.
Objectives: The present investigation is focused to find out chemotypic variation of lupeol in
roots of Hemidesmus indicus collected from twelve locations belonging to ten agroclimatic
regions of Madhya Pradesh state of India for its scientific validation, quality standardization
and selection of elite chemotypes.
Methods: 1 gm powdered root sample of each location was refluxed in methanol for 15-20
minutes consecutively three times on a water bath. The extract solutions were pooled, filtered
and concentrated to get the extract residues. Dried extracts were dissolved separately in
10 ml of methanol to prepare the test samples to assess lupeol content using CAMAG
HPTLC system equipped with Linomat V applicator, TLC scanner, WIN CATS software
Version 1.4.8 and Hamilton Syringe (100 μL).
Results: The result showed significant variation in lupeol content in the range from 0.016 to
0.299% (dry wt.). The highest content of lupeol (0.299%) was found in the accessions of
Amarkantak and lowest content (0.016%) in the accessions of TFRI belonging to Northern
Hills Zone of Chhattisgarh and Kymore Plateau & Satpura Hills agroclimatic regions of
Madhya Pradesh, respectively.
Conclusion: Variation in lupeol content resulted from the study revealed the accessions of
Amarkantak of best quality and elite chemotypes which may be in situ conserved for their
further exploitation to meet the demand of the species on commercial scale.