Quantification of Angiotensins: Is there a Consensus in the Literature?
Pp. 337-358 (22)
Zaira Palomino Jara, Andreia Cristina Febba Gomes, Fernanda Barrinha Fernandes, Dale M Seth, Minolfa C. Prieto, L. Gabriel Navar and Dulce Elena Casarini
The renin angiotensin system (RAS) is an essential regulator of renal and
cardiovascular function. The components of this system are present in the circulation,
organs, tissues and various cell types; and their syntheses in the organs are independent
of the circulation. Understanding how the circulatory and organ RAS interact in the
maintenance of the body function is knowledge that is not only key, but absolutely
essential in helping us to unravel the implications of the RAS in a disease process. In
this regard, this chapter aims to verify whether or not there is a consensus in the
literature for the reported quantities and concentrations of Angiotensin I (Ang I),
Angiotensin II (Ang II) and Angiotensin 1-7 (Ang1-7) in normal physiological states in
mice, rats and humans. Because of the various methods for quantification of
angiotensins, there is not presently a clear consensus for the absolute quantities of these
peptides in plasma and the different tissues.
Angiotensins, Angiotensin converting enzyme (ACE), Angiotensin
converting enzyme 2 (ACE2), Angiotensin I, Angiotensin II, Angiotensin 1-7, Consensus, Enzyme-linked immunosorbent assay (ELISA), Extraction of angiotensins,
High performance liquid chromatography (HPLC), Liquid chromatography (LC) mass
spectrometry (MS), Plasma angiotensins, Protease inhibitors, Radioimmunoassay (RIA),
Rua Botucatu, 740 - 2° andar, Department of Medicine/Nephrology Division, Vila Clementino - Sao Paulo/SP, Brazil, CEP 04023-900.