Immunogenicity and Therapeutic Effects of pVAX1-rv1419 DNA from Mycobacterium tuberculosis
Background: Tuberculosis (TB) is threatening disease in China and new therapeutic agents
and regimens to treat TB are urgently needed.
Objective: In this study, a DNA vaccine expressing Mycobacterium tuberculosis (MTB) Rv1419 antigen
was constructed and its immunogenicity and therapeutic effects were evaluated.
Method: Normal mice and TB model mice were immunized intramuscularly three times at two-week
intervals with saline, plasmid vector pVAX1, M. vaccae vaccine, pVAX1- ag85a (rv3804c) DNA or
pVAX1-rv1419 DNA, respectively.
Results: At three weeks after the last immunization, flow cytometry showed a higher proportion of
T cells expressing IFN-γ (Th1) in response to Rv1419 protein in blood from the pVAX1- rv1419
DNA group compared with the saline and vector groups (P<0.05), suggesting a predominant Th1 immune
response. Live bacterial loads in lungs and spleens were lower by 0.41 log10 in the pVAX1-
rv1419 DNA group than in the saline controls. In addition, pathological changes in the lungs of the
DNA vaccinated groups were less. These results suggest that pVAX1- rv1419 DNA could be effective
for the treatment of TB, significantly increasing the Th1-type cellular immune response, and inhibiting
the growth of MTB.
Conclusion: Therefore pVAX1- rv1419 DNA is a candidate for inclusion in a therapeutic combination
DNA vaccine against TB.
Keywords: DNA vaccine, pVAX1-rv1419 DNA, Immunotherapy, Mycobacterium tuberculosis, Rv1419 protein, Immunization.
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