Background: Antifouling paints are widely used for the prevention of growth and development
of fouling organisms (biofouling). Consequently, nowadays they are one of the main sources of
Objective: The present study examines and compares two different extraction techniques, for the determination
of diuron and irgarol 1051 in shellfish tissues.
Method: Microwave assisted extraction and ultrasound extraction, followed by liquid chromatography
with tandem mass spectrometry were tested and different parameters for both extraction methods were
Results: The results showed that microwave assisted extraction was the most effective method, presenting
recoveries in the range of 51.2 to 69.4% and 76.0 to 92.8% for diuron and irgarol 1051, respectively.
The precision was evaluated in terms of repeatability for different concentration levels (50, and 500
ng·g-1 of both analytes), which was lower than 19.1% (n = 6) in all cases. The applicability of the proposed
method was evaluated by the extraction and determination of the target analytes in different mollusc
species collected from Spain and Brazil beaches.
Conclusion: The proposed method provides good results in terms of sensitivity, extraction efficiencies
and precision and it could be employed for future monitoring studies in ecosystems influenced by the