Background: High doses of Cisplatin (CP) can disrupt the normal functioning of various
tissues such as ovaries and testis. In almost all the patients, spermiotoxicity of CP causes temporary or
Objective: In this study, the defensive effect of Melissa ofcinalis and vitamin E against testicular injuries
caused by CP in male rats was evaluated.
Method: Thirty six male rats were distributed into 6 groups. Group 1 was used as the negative control. In
group 2, a single dose of CP (10 mg/kg) was administered on the first day. In groups 3 and 4, a single
dose of CP (10 mg/kg) was administered on the first day and then treated with Melissa ofcinalis at 1000
mg/kg/day and vitamin E at 100 mg/kg/day for 7 consecutive days, respectively. Groups 5 and 6 were
treated with Melissa ofcinalis and vitamin E for 7 consecutive days, respectively. After euthanasia, serum
levels of testosterone, Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) were
evaluated. Testes were removed and weighed. Spermatic analysis was done on the tail of the epididymis.
Tissue lipid peroxidation and activity of antioxidant enzymes in testes were evaluated as well.
Results: The results showed that Melissa ofcinalis and vitamin E increased serum levels of testosterone,
LH and FSH, weight of testes and sperm motility, count and vitality and decreased sperm cell abnormalities
in rats given CP.
Conclusion: Our results are useful in designing a medication of Melissa ofcinalis that can protect the
testes against CP-induced testicular damage and infertility in cancerous male patients.