Antibacterial Effect of Gallic Acid against Aeromonas hydrophila and Aeromonas sobria Through Damaging Membrane Integrity

Title:Antibacterial Effect of Gallic Acid against Aeromonas hydrophila and Aeromonas sobria Through Damaging Membrane Integrity

Volume: 17 Issue: 13

Author(s): Jing Lu, Zhenning Wang, Mengrou Ren, Guoren Huang, Baochen Fang, Xiujuan Bu, Yanhui Liu and Shuang Guan

Affiliation:

Keywords: Gallic acid, Aeromonas hydrophila, Aeromonas sobria, antibacterial activity, membrane integrity, cell morphology.

Abstract: In the study, we investigated the antibacterial activity and mechanism of gallic acid against Aeromonas hydrophila and Aeromonas sobria. Gallic acid showed strong antimicrobial activity against the two bacteria. Furthermore, the antibacterial mechanism of gallic acid (0, 3, 6, 12 mM) was performed by membrane integrity assay and scanning electron microscopy (SEM) assay. The results showed that gallic acid notably increased the released material absorption value at 260, 280 nm and electric conductivity in a dose-dependent manner. Moreover, the SEM assay showed that gallic acid induced severe shrink of bacterial intima and irregular morphology in a dose-dependent manner. The SDS-PAGE profiles further confirmed that gallic acid could damage bacterial cells. These results indicated gallic acid exhibited antibacterial effect by destroying membrane integrity of A. hydrophila and A. sobria. Hence, gallic acid has great potential as a new natural food preservative in food fresh-keeping and storage.

Cite this article as:

Lu Jing, Wang Zhenning, Ren Mengrou, Huang Guoren, Fang Baochen, Bu Xiujuan, Liu Yanhui and Guan Shuang, Antibacterial Effect of Gallic Acid against Aeromonas hydrophila and Aeromonas sobria Through Damaging Membrane Integrity, Current Pharmaceutical Biotechnology 2016; 17 (13) . https://dx.doi.org/10.2174/1389201017666161022235759