Introduction: Chromatographic methods for determination of betamethasone or dexamethasone
in their pharmaceutical preparations by Ultra Performance Liquid Chromatography-Tandem Mass
Spectrometry were developed.
Method: Analytes were eluted from Acquity BEH C18 (50 mm×2.1 mm, 1.7 µm) column using stepwise
gradient with mobile phase consisted of acetonitrile and 0.1% (v/v) acetic acid in water. Quantitation of
dexamethasone and betamethasone was performed using 451>361 m/z on MRM mode.
Results: Methods were linear over the concentration range from 10 to 1500 ng mL
-1 for both analyte.
Limits of detection for BTM or DXM were 1 ng mL
-1. The highest relative error and relative standard
deviation values were not over 2%.
Conclusion: Presented methods were specific, rugged and robust, successfully applied to the analysis of
their pharmaceutical preparations.