Background: The current study was performed to evaluate the anti-urolithiatic activity of
Argemone mexicana L. leaves extracts as natural remedy to over come side effects problem due to the
usage of modern synthetic medicine.
Methods: In vitro experiments (nucleation, aggregation and microscopic assay’s) were performed to test
the anti-urolithiasis activity of A. mexicana extracted using the four different solvents i.e. petroleum
ether, chloroform, methanol, and water. These extracts were tested at increasing concentration of 25, 50,
75 and 100 mg/ml and compared with the standard drug cystone.
Result and Discussion: The extracts were tested for the inhibition percentage of anti-urolithiasis potential
against preformed calcium oxalate crystal. The results of A. mexicana extracts on nucleation assay were
found to be significant and the methanol leaf extract showed considerable inhibition (72.26%) compared to
standard cystone drug (62.96%) and in aggregation assay also the methanol leaf extract of A. mexicana
showed significant inhibition (77.24%) compared to cystone (69.33%) at 100 mg/ml concentration. The
microscopic assay clearly indicated that A. mexicana leaves extracts are capable to dissolve the calcium
Conclusion: The methanol extract of A. mexicana leaves contains anti-urolithiatic potentials by in vitro at
a dose level of 100 mg/ml. The methanol extract of A. mexicana leaves can be further exploited as a
potential anti-urolithiasis drug.