Abstract
Background: Many studies suggest oxidative stress as an early feature of Alzheimer’s Disease (AD). However, evidence of established oxidative stress in AD peripheral cells is still inconclusive, possibly due to both, differences in the type of samples and the heterogeneity of oxidative markers used in different studies.
Objective: The aim of this study was to evaluate blood-based redox alterations in Alzheimer’s Disease in order to identify a peculiar disease profile. Method: To that purpose, we measured the activity of Superoxide Dismutase, Catalase and Glutathione Peroxidase both in the extracellular and the intracellular blood compartments of AD, MCI and control subjects. The amount of an open isoform of p53 protein (unfolded p53), resulting from oxidative modifications was also determined. Results: Decreased SOD, increased GPx activity and higher p53 open isoform were found in both AD and MCI plasma compared to controls. In blood peripheral mononuclear cells, SOD activity was also decreased in both AD and MCI, and unfolded p53 increased exquisitely in younger AD males compared to controls. Conclusion: Overall, these data highlight the importance of considering both extracellular and intracellular compartments, in the determination of antioxidant enzyme activities as well as specific oxidation end-products, in order to identify peculiar blood-based redox alterations in AD pathology.Keywords: Alzheimer's disease, antioxidant enzymes, cognitive decline, mild cognitive impairment, redox alterations, unfolded p53.
Current Alzheimer Research
Title:Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients
Volume: 14 Issue: 1
Author(s): Noemí Arce-Varas, Giulia Abate, Chiara Prandelli, Carmen Martínez, Fernando Cuetos, Manuel Menéndez, Mariagrazia Marziano, David Cabrera-García, María Teresa Fernández-Sánchez, Antonello Novelli, Maurizio Memo and Daniela Uberti
Affiliation:
Keywords: Alzheimer's disease, antioxidant enzymes, cognitive decline, mild cognitive impairment, redox alterations, unfolded p53.
Abstract: Background: Many studies suggest oxidative stress as an early feature of Alzheimer’s Disease (AD). However, evidence of established oxidative stress in AD peripheral cells is still inconclusive, possibly due to both, differences in the type of samples and the heterogeneity of oxidative markers used in different studies.
Objective: The aim of this study was to evaluate blood-based redox alterations in Alzheimer’s Disease in order to identify a peculiar disease profile. Method: To that purpose, we measured the activity of Superoxide Dismutase, Catalase and Glutathione Peroxidase both in the extracellular and the intracellular blood compartments of AD, MCI and control subjects. The amount of an open isoform of p53 protein (unfolded p53), resulting from oxidative modifications was also determined. Results: Decreased SOD, increased GPx activity and higher p53 open isoform were found in both AD and MCI plasma compared to controls. In blood peripheral mononuclear cells, SOD activity was also decreased in both AD and MCI, and unfolded p53 increased exquisitely in younger AD males compared to controls. Conclusion: Overall, these data highlight the importance of considering both extracellular and intracellular compartments, in the determination of antioxidant enzyme activities as well as specific oxidation end-products, in order to identify peculiar blood-based redox alterations in AD pathology.Export Options
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Cite this article as:
Arce-Varas Noemí, Abate Giulia, Prandelli Chiara, Martínez Carmen, Cuetos Fernando, Menéndez Manuel, Marziano Mariagrazia, Cabrera-García David, Fernández-Sánchez Teresa María, Novelli Antonello, Memo Maurizio and Uberti Daniela, Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients, Current Alzheimer Research 2017; 14 (1) . https://dx.doi.org/10.2174/1567205013666161010125413
DOI https://dx.doi.org/10.2174/1567205013666161010125413 |
Print ISSN 1567-2050 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5828 |
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Aims and Scope: Introduction: Alzheimer's disease (AD) poses a significant global health challenge, with an increasing prevalence that demands concerted efforts to advance our understanding and strategies for prevention, diagnosis, treatment, and rehabilitation. This thematic issue aims to bring together cutting-edge research and innovative approaches from multidisciplinary perspectives to address ...read more
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Neuroinflammation is an invariable hallmark of chronic and acute neurodegenerative disorders and has long been considered a potential drug target for Alzheimer?s disease (AD) and dementia. Significant evidence of inflammatory processes as a feature of AD is provided by the presence of inflammatory markers in plasma, CSF and postmortem brain ...read more
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Alzheimer's disease (AD) poses a significant global health challenge, with an increasing number of individuals affected yearly. Deep learning, a subfield of artificial intelligence, has shown immense potential in various domains, including healthcare. This thematic issue of Current Alzheimer Research explores the application of deep learning techniques in advancing our ...read more
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Dementia affects 18 million people worldwide. Dementia is a syndrome of symptoms caused by brain disease, usually chronic or progressive, clinically characterized by multiple impairments of higher cortical functions such as memory, thinking, orientation, and learning. In addition, in the course of dementia, cognitive deficits are observed, which often hinder ...read more
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