Background: Mesial temporal lobe epilepsy is the most prevalent type of human epilepsy
and its pathogenesis still remains unknown. Structures outside the temporal lobe may also play critical
roles in the disease’s progress.
Objective: The aim of this study was to investigate proteome alterations and to identify differentially
expressed proteins in frontoparietal cortex and thalamus regions of 6-month-old amygdala-kindled
WAG/Rij rats as a mesial temporal lobe epilepsy model by using bottom-up proteomics approach.
Method: Protein extraction from the tissues was followed by two-dimensional gel electrophoresis.
Proteins were identified by peptide mass fingerprinting analysis using MALDI-TOF MS followed by
MASCOT database search.
Results: 58 and 47 proteins were identified in frontoparietal cortex and thalamus, respectively. Differentially
expressed proteins in frontoparietal cortex were all up-regulated in the kindled groups
compared to kindled-resistant group (p<0.05). These proteins were; Fabp4, Gamma-enolase, Annexin
AI, Rab-15, RAB6-interacting golgin, PGAM1, DAB-2 and Fructose-bisphosphate aldolase C.
In thalamus, BDNF (in spot 13), TRAPPC2L, Ras-related protein Rab-2A, GTP-binding protein
REM 2 and Calcyclin-binding protein were up-regulated (p<0.05); and BDNF (in spot 9), kif3a, Parvalbumin
alpha were down-regulated in the kindled groups compared to the kindled-resistant group
Conclusion: In this study, we identified proteins that might have roles in enabling or complicating
mesial temporal lobe epilepsy progress. The potential of these proteins as biomarkers needs further