Analytical Method Development and Validation for Concurrent Determination of Bromohexine and Phenylpropanolamine by Reverse Phase-HPLC Method

Title:Analytical Method Development and Validation for Concurrent Determination of Bromohexine and Phenylpropanolamine by Reverse Phase-HPLC Method

Volume: 13 Issue: 5

Author(s): Kishor Kumar Erukulla and S. R. Suseem*

Affiliation:

• Department of Chemistry, VIT University, Vellore, Tamilnadu, 632014,India

Keywords: Bromohexine, phenylpropanolamine, validation, method, buffer and development.

Abstract: Objective: A particular, easy and precise reverse phase liquid chromatographic technique was developed for the synchronous determination of Bromohexine and Phenylpropanolamine in pharmaceutical dose forms as there is no consolidated strategy accessible for the concurrent determination of Bromohexine and Phenylpropanolamine.

Methods: The BDS C18 (250mm x 4.6mm, 5µm) column was utilized in isocratic mode. The mobile phase contains phosphate buffer and acetonitrile (50:50 v/v). The buffer is set up by including 1ml of orthophosphoric acid in a 1000 ml of volumetric flask and 900 ml of milli-Q water added and degas to sonicate lastly make up the volume with water. The rate of flow was 1 ml/min. The effluents were observed at 250 nm.

Results:The observed retention time of Bromohexine was 2. 323 min and for Phenylpropanolamine 2. 719 min. The linearity values observed for bromohexine was of 8-48 µg/ml and for phenylpropanolamine, it was 25-150 µ g/ml. The observed recovery of Bromohexine was 98. 16 % and phenylpropanolamine was 101.29 %.

Conclusion: The proposed strategy was validated and the same is very useful for the synchronous determination of Bromohexine and Phenylpropanolamine.

Cite this article as:

Erukulla Kumar Kishor and Suseem R. S.*, Analytical Method Development and Validation for Concurrent Determination of Bromohexine and Phenylpropanolamine by Reverse Phase-HPLC Method, Current Pharmaceutical Analysis 2017; 13 (5) . https://dx.doi.org/10.2174/1573412912666161004144334

DOI https://dx.doi.org/10.2174/1573412912666161004144334	Print ISSN 1573-4129
Publisher Name Bentham Science Publisher	Online ISSN 1875-676X