Reactivation of latent HIV-1 in latently infected cells by coumarin compounds: Hymecromone and ScoparoneReactivation of Latent HIV-1 in Latently Infected Cells by Coumarin Compounds: Hymecromone and Scoparone

Author(s): Xian Li, Hanxian Zeng, Pengfei Wang, Lu Lin, Lin Liu, Pinyi Zhen, Yuanzhe Fu, Panpan Lu, Huanzhang Zhu*.

Journal Name: Current HIV Research

Volume 14 , Issue 6 , 2016

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Abstract:

Background: Current antiretroviral therapy (ART) cannot cure HIV-1 infection due to the presence of latent viral reservoirs. The “shock and kill” strategy is a promising approach to eliminate the viral reservoir. However, there are various limits existing in current latency-reversing agents, searching for new activators are urgently needed.

Objective: The present study aimed at investigating the ability of hymecromone and scoparone for activating HIV-1 from latent reservoirs.

Methods: Jurkat T cell model of HIV-1 latently were used to evaluate the effect of hymecromone and scoparone. The percentage of green florescence protein expression as a marker for reactivation of HIV-1 promoter was measured via FACScan. The expression of CD25 and CD69 in human peripheral blood mononuclear cells was measured by flow cytometry at 72 h post-treatment with hymecromone or scoparone or prostratin using antibodies against CD25 and CD69.

Results: Hymecromone and scoparone can induce HIV-1 LTR reactivation in a dose and timedependent. We further show that hymecromone and scoparone can reactivate latent virus without inducing the activation of global T cells. We also found that scoparone acts by NF-&kgr;B signal pathway.

Conclusion: Hymecromone and scoparone can effectively reactivate latent HIV-1 with low cellular toxicity, indicating hymecromone and scoparone might be potential drugs for HIV-1 reservoir eradication strategies in the future.

Keywords: Coumarin compounds, hymecromone, scoparone, HIV-1 latency, reactivation.

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Article Details

VOLUME: 14
ISSUE: 6
Year: 2016
Page: [484 - 490]
Pages: 7
DOI: 10.2174/1570162X14666161003152458
Price: $58

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