Background: Chronic Kidney Disease (CKD) is an independent risk factor for cardiovascular
disease (CVD). CKD is accompanied by high cardiovascular mortality due to many factors, but atherosclerosis
is thought to be a major cause at every CKD stage. It has been suggested that measuring and
estimating changes in high density lipoprotein (HDL) and low density lipoprotein (LDL) subfractions
may be important for predicting CVD in CKD patients.
Objective: The aim of this study was to determine and compare levels of HDL and LDL subfractions in
patients with different CKD stages.
Methods: The study included 115 patients with CKD (CKD stage 2-25 patients, CKD stage 3-25; CKD
stage 4-25 and CKD 5 undergoing dialysis - 40 patients) and 25 volunteers without CKD (control group).
The Lipoprint System (Quantimetrix®) was used to analyse HDL and LDL subfractions.
Results: There were significant differences in the distribution of HDL1-HDL5 subfractions levels, which
were significantly higher in patients with impaired renal function than in the control group (p≤0.013 for
all comparisons). HDL7-HDL10 subfractions were significantly more prevalent in healthy volunteers
compared with CKD patients (p≤0.001 for all comparisons). The analysis of LDL subfractions revealed
significant differences only in IDL-B (p<0.05), IDL-A (p<0.05) and LDL2 (p<0.001) between patients
with CKD stage 5 and controls.
Conclusion: CKD influenced HDL and LDL subfractions. In CKD patients, large HDL subpopulations
were more prevalent in contrast to small HDL subfractions in healthy subjects. Identification of patients
with increased level of large HDL subfractions could be useful to identify CKD subjects at increased CV
risk. Further studies with larger populations and with the application of a several methods of subfraction
measurement are necessary to confirm these results.