Objective: The most used techniques to evaluate the inhibitor activity of different compounds
on Nitric Oxide Synthases are presented.
Method: Griess assay, oxy-hemoglobin capture assay, Radio citrulline assay, and RP-HPLC assay.
Result: Griess assay, and oxy-hemoglobin capture assay detect the nitric oxide produced by NOS;
while radio citrulline assay, and RP-HPLC assay take into consideration the concentration of Lcitrulline
as stoichiometric to nitric oxide.
Conclusion: Griess assay and oxy-hemoglobin capture assay are suitable for a first screening of NOS
activity, while the others could be used for a more precise activity determination.