Methods to Evaluate the Activity of Nitric Oxide Synthase

Author(s): Marialuigia Fantacuzzi*, Alessandra Ammazzalorso, Barbara De Filippis, Letizia Giampietro, Cristina Maccallini, Rosa Amoroso.

Journal Name: Current Pharmaceutical Analysis

Volume 13 , Issue 5 , 2017

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Abstract:

Objective: The most used techniques to evaluate the inhibitor activity of different compounds on Nitric Oxide Synthases are presented.

Method: Griess assay, oxy-hemoglobin capture assay, Radio citrulline assay, and RP-HPLC assay.

Result: Griess assay, and oxy-hemoglobin capture assay detect the nitric oxide produced by NOS; while radio citrulline assay, and RP-HPLC assay take into consideration the concentration of Lcitrulline as stoichiometric to nitric oxide.

Conclusion: Griess assay and oxy-hemoglobin capture assay are suitable for a first screening of NOS activity, while the others could be used for a more precise activity determination.

Keywords: Nitric oxide synthase, nitric oxide, L-arginine, L-citrulline, griess assay, hemoglobin assay, citrulline assay, RPHPLC assay.

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Article Details

VOLUME: 13
ISSUE: 5
Year: 2017
Page: [411 - 416]
Pages: 6
DOI: 10.2174/1573412912666160916154405
Price: $58

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