Abstract
Objective: The most used techniques to evaluate the inhibitor activity of different compounds on Nitric Oxide Synthases are presented.
Method: Griess assay, oxy-hemoglobin capture assay, Radio citrulline assay, and RP-HPLC assay. Result: Griess assay, and oxy-hemoglobin capture assay detect the nitric oxide produced by NOS; while radio citrulline assay, and RP-HPLC assay take into consideration the concentration of Lcitrulline as stoichiometric to nitric oxide. Conclusion: Griess assay and oxy-hemoglobin capture assay are suitable for a first screening of NOS activity, while the others could be used for a more precise activity determination.Keywords: Nitric oxide synthase, nitric oxide, L-arginine, L-citrulline, griess assay, hemoglobin assay, citrulline assay, RPHPLC assay.
Current Pharmaceutical Analysis
Title:Methods to Evaluate the Activity of Nitric Oxide Synthase
Volume: 13 Issue: 5
Author(s): Marialuigia Fantacuzzi*, Alessandra Ammazzalorso, Barbara De Filippis, Letizia Giampietro, Cristina Maccallini and Rosa Amoroso
Affiliation:
- Department of Pharmacy, “G. d’Annunzio” University, Chieti,Italy
Keywords: Nitric oxide synthase, nitric oxide, L-arginine, L-citrulline, griess assay, hemoglobin assay, citrulline assay, RPHPLC assay.
Abstract: Objective: The most used techniques to evaluate the inhibitor activity of different compounds on Nitric Oxide Synthases are presented.
Method: Griess assay, oxy-hemoglobin capture assay, Radio citrulline assay, and RP-HPLC assay. Result: Griess assay, and oxy-hemoglobin capture assay detect the nitric oxide produced by NOS; while radio citrulline assay, and RP-HPLC assay take into consideration the concentration of Lcitrulline as stoichiometric to nitric oxide. Conclusion: Griess assay and oxy-hemoglobin capture assay are suitable for a first screening of NOS activity, while the others could be used for a more precise activity determination.Export Options
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Cite this article as:
Fantacuzzi Marialuigia*, Ammazzalorso Alessandra, De Filippis Barbara, Giampietro Letizia, Maccallini Cristina and Amoroso Rosa, Methods to Evaluate the Activity of Nitric Oxide Synthase, Current Pharmaceutical Analysis 2017; 13 (5) . https://dx.doi.org/10.2174/1573412912666160916154405
DOI https://dx.doi.org/10.2174/1573412912666160916154405 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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