Stem Cells Between Regeneration and Tumorigenesis

Stem Cells Between Regeneration and Tumorigenesis

Indexed in: EBSCO.

Experts in the field of cellular biology have shown that the reactivation of pluripotency inherent in all cells can allow us to reprogram cells into a specific cell line. This reprogramming paradigm ...
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Nano and Microtechnology for Monitoring Stem Cell Differentiation

Pp. 281-320 (40)

Larisa-Emilia Cheran, Alin Cheran, Andreea-Roxana Lupu and Traian Popescu

Abstract

This chapter gives an overview of the new micro- and nano-technologies designed to monitor SC differentiation in the context of their potential applications in disease modeling, tissue engineering, regenerative medicine, as well as drug screening and toxicology. Representative examples of such applications are presented and the crucial importance of the differentiation processes for the safety of SC therapies is discussed. The roles of the main factors that influence SC differentiation are briefly summarized and the vital need to control and monitor the differentiation process using non-invasive methods is emphasized. The basic principles of new micro- and nanotechnologies for monitoring SC differentiation are presented, with special focus on the use of acoustic vibrational fields to characterize SC. Literature studies on SC differentiation, involving methods based on Impedance Sensing (IS), Surface Enhanced Raman Spectroscopy (SERS), Fourier Transformed Infrared (FTIR) spectroscopy, Microelectrode Array (MEA) sensors, Light-addressable Potentiometric Sensors (LAPS), Field-effect Transistors (FET), Surface Plasmon Resonance (SPR) and Quartz Crystal Microbalance (QCM), are briefly described.

Keywords:

Differentiation, Monitoring, Nano and microtechnology, Stem cells.

Affiliation:

Department of Chemistry, University of Toronto, Toronto, Ontario, Canada.