Background: Glycyrrhetinic acid ammonium (GAA) inhibits the
biosynthesis of prostaglandins PGE-2 and PGF-2α to their inactive
metabolites. This causes an increased level of prostaglandins in the digestive
system which inhibit gastric secretion.
Objective: The aim of the present investigation was to develop mucoadhesive
microspheres of GAA using Colocasia esculenta (CE) and Bombax ceiba (BC)
mucilages, alone and in combination for the efficient treatment of gastric ulcer.
Methods: The glycyrrhetinic acid (GA) was extracted as ammonium salt
from dried stolons of Glycyrrhiza glabra using hot maceration technique.
Extraction of mucilage was carried out using herbs and rhizomes of CE and flowers of BC by
cold maceration method. The hydrophilic molecule of GAA was encapsulated using single phase
emulsification technique. Glutaraldehyde was used as cross-linking agent. The developed microspheres
were evaluated for morphology, particle size, entrapment efficiency, drug loading, in
vitro mucoadhesion, swelling behavior, in vitro drug release and in vivo ulcer healing activity.
Results: The results showed that the drug-polymer in 1: 2 ratio in CE- mucilage containing
microspheres exhibited prolonged drug release as compared to BC-mucialge or their
combination. The stability study results showed stable character of GAA in the developed
microspheres. The developed microspheres show effective in vivo ulcer healing activity in
Wistar male albino rats.
Conclusion: The preliminary results of this study suggest that the developed mucoadhesive
microspheres containing GAA could be a promising tool in the treatment of gastric ulcer and
can be advantageous over the conventional anti ulcer therapy. The study also suggested that the
extracted mucilages can be used in the preparation of the mucoadhesive microspheres.