Site-directed mutagenesis allows elucidation of the basic principles of the porin-driven
membrane permeability and opens the possibility for the modulation of functional states of porin channels.
The review is aimed to show the advantages of using mutant and chemically modified porins for
obtaining detailed information about molecular mechanisms that underlie the non-specific transmembrane
diffusion. We summarized data regarding the effects of the point substitutions and the external
loop deletions on electrophysiological properties of general porins. The influence of charges inside the
pore eyelet and the roles of external loops in ion conductance, ion selectivity, and voltage gating were
Keywords: Outer membrane porins, electrophysiological properties, site-directed mutagenesis, point substitutions and loop
deletions, pore selectivity, voltage gating.
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