Wound Healing Activity of Hydro-alcoholic Extract of Cinnamomum nitidum Blume (Lauraceae) in Wistar Albino Rats

Author(s): Chaudhary Deepa, Rajnish Srivastava, Amit Kumar Srivastava, Anamika Kotiya.

Journal Name: Current Traditional Medicine

Volume 2 , Issue 2 , 2016

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Graphical Abstract:


Aim: The present study was conducted with an objective to investigate the wound healing effect of hydro-alcoholic extract of stem bark of Cinnamomum nitidum blume (HECNB).

Methods: The total polyphenols and total flavonoid content were estimated by Folin-Ciocalteu and aluminum chloride methods. The antioxidant activity was assessed by DPPH method. Wistar albino rats were grouped into; group I (left untreated) considered as control, group II (Povidone Iodine 5% w/w, once daily), considered as standard, group III (HECNB 2% w/w, once daily) and group IV (HECNB 5% w/w, once daily), were considered as test groups. The wound healing effect was assessed as percentage healing, wound breaking strength and hydroxyproline level in the incision wound model. % wound contraction, epithelization period and histopathological examinations were assessed in the excision wound model.

Result: HECNB exhibits maximum free radical scavenging activity of 53.11% as compared to standard ascorbic acid (82.13%). HECNB in different concentrations (2% and 5% w/w) significantly potentiate the healing process in both the models. HECNB showed dose-dependent percentage wound healing (P < 0.01). Significant wound contraction was found in HECNB treated groups as compared with control (P < 0.01) animals. High degree of tensile strength in HECNB treated animals was found (P < 0.01) as compared with control. Hydroxyproline level was found to be significantly increased (P < 0.01) in HECNB treated animals in a dose-dependent manner. Histopathological studies also revealed the effectiveness of HECNB in improved wound healing.

Conclusion: Hydro-alcoholic extract of stem bark extract of Cinnamomum nitidum blume (HECNB) possesses concentration dependent wound healing effect.

Keywords: Cinnamomum nitidum blume, DPPH, Hydroxyproline, Folin-Ciocalteu, hydroxyproline, incision wound, wound excision, wound healing.

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Article Details

Year: 2016
Page: [134 - 145]
Pages: 12
DOI: 10.2174/2215083802666160902154451
Price: $58

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