Development and Validation of a Simple High-Performance Liquid Chromatography Method for Estimation of Felodipine in Rabbit Plasma: Application to Pharmacokinetic Study

Author(s): Murali Monohar Pandey, Shrikant Y. Charde*, A. Jaipal, Prashant P. Raut, Puneet Goel, Love Kumar.

Journal Name: Current Pharmaceutical Analysis

Volume 13 , Issue 4 , 2017

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Abstract:

Background: A new, simple, specific and sensitive reverse-phase liquid chromatographic method was developed and validated for the estimation of felodipine (FDP) in rabbit plasma using fluorescence detector.

Materials and Methods: Plasma standard were prepared using a simple and one-step protein precipitation method and 50 μ l of the standard solutions were injected into the column. The method was found to be linear in the concentration range of 10–1000 ng/ml. The developed bioanalytical method was validated as per standard guidelines. Validation study results demonstrated accuracy, precision, sensitivity and selectivity of the proposed method.

Results: The developed method was successfully employed in the determination of FDP in plasma samples obtained from the pharmacokinetic study of the immediate release tablets in rabbits.

Keywords: Felodipine, fluorescence detector, high-performance liquid chromatography, rabbit plasma, pharmacokinetic study, oral tablet.

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Article Details

VOLUME: 13
ISSUE: 4
Year: 2017
Page: [321 - 327]
Pages: 7
DOI: 10.2174/1573412912666160824120801
Price: $58

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