Background: Topical drug delivery system is an attractive way to target
dermatological disorders. Dapsone is commonly used as anti-leprotic drug. In this research
work dapsone was repurposed for treating acne via topical application.
Objective: The aim of present work was to formulate, optimize and characterize hybrid
nanoparticles (HNs) containing DAP (dapsone) and incorporating the same into a topical gel
Method: The HNs were prepared using melt-emulsification and ultrasonication process. The
HN formulations were characterized for particle size, zeta potential and entrapment efficiency
applying 23 factorial design.
Results: The results revealed that HN-4 has an average particle size of 277nm, zeta potential of
-26.7 mV, entrapment efficiency of 75.81%. Transmission electron microscopy (TEM)
confirmed that HNs were spherical in shape with polymer core surrounded by a lipid shell.
Further, the pelletized HNs were incorporated in carbopol 940 gel base and analysed for pH,
drug content, viscosity and optimized batch was evaluated for texture analysis, in-vitro skin
permeation, and antimicrobial study. The stability studies showed that DAP-HN4 G3 (dapsone
loaded carbopol gel) stored at 40±2oC was stable and non-irritant as per Hen’s Egg Test
Chorioallantoic Membrane (HET CAM) study.
Conclusion: It can be concluded that formulation containing DAP HNs was found to be stable,
non-irritant and may prove to be an effective and promising delivery system for acne