Antiproliferative and Apoptotic Effect of Dendrosomal Curcumin Nanoformulation in P53 Mutant and Wide-Type Cancer Cell Lines

Author(s): Maryam Montazeri , Younes Pilehvar-Soltanahmadi , Mina Mohaghegh , Alireza Panahi , Samaneh Khodi , Nosratollah Zarghami* , Majid Sadeghizadeh* .

Journal Name: Anti-Cancer Agents in Medicinal Chemistry

Volume 17 , Issue 5 , 2017

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Abstract:

Objective: The aim of this paper is to investigate the effect of dendrosomal curcumin (DNC) on the expression of p53 in both p53 mutant cell lines SKBR3/SW480 and p53 wild-type MCF7/HCT116 in both RNA and protein levels.

Background: Curcumin, derived from Curcumin longa, is recently considered in cancer related researches for its cell growth inhibition properties. p53 is a common tumor-suppressor gene involved in cancers and its mutation not only inhibits tumor suppressor activity but also promotes oncogenic activity.

Method: Here, p53 mutant/Wild-type cells were employed to study the toxicity of DNC using MTT assay, Flow cytometry and Annexin-V, Real-time PCR and Western blot were used to analyze p53, BAX, Bcl-2, p21 and Noxa changes after treatment.

Results: During the time, DNC increased the SubG1 cells and decreased G1, S and G2/M cells, early apoptosis also indicated the inhibition of cell growth in early phase. Real-Time PCR assay showed an increased mRNA of BAX, Noxa and p21 during the time with decreased Bcl-2. The expression of p53 mutant decreased in SKBR3/SW480, and the expression of p53 wild-type increased in MCF7/HCT116.

Conclusion: Consequently, p53 plays an important role in mediating the survival by DNC, which can prevent tumor cell growth by modulating the expression of genes involved in apoptosis and proliferation.

Keywords: Dendrosomal curcumin (DNC), p53 mutant, apoptosis, breast cancer, colon cancer.

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Article Details

VOLUME: 17
ISSUE: 5
Year: 2017
Page: [662 - 673]
Pages: 12
DOI: 10.2174/1871520616666160815124537
Price: $58

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