Expression of Odontogenic and Osteogenic Markers in DPSCs and SHED: A Review

Author(s): Hii Siew Ching, Norhayati Luddin, Ismail Ab Rahman, Kannan Thirumulu Ponnuraj.

Journal Name: Current Stem Cell Research & Therapy

Volume 12 , Issue 1 , 2017

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Abstract:

The odontogenic and osteogenic potential of dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous tooth (SHED) have been shown clearly by various in vitro and in vivo studies. The findings are promising and demonstrated that dental tissue engineering can give a new hope to the individuals suffering from tooth loss and dental diseases. The evaluation of odontogenic and osteogenic differentiation of DPSCs and SHED is commonly carried out by an illustration of the expression of varied related markers. In this review, few commonly used markers such as alkaline phosphatase (ALP), collagen type 1 (Col I), dentin matrix acid phosphoprotein 1 (DMP1), dentin sialophosphoprotein (DSPP), matrix extracellular phosphoglycoprotein (MEPE), osteocalcin (OCN), and osteopontin (OPN). DSPP, DMP1, and MEPE (odontogenic markers), which play an important role during early odontoblastic differentiation and late dentin mineralization, have been highlighted. Osteoblastic proliferation and early/late osteoblastic differentiation can be assessed by estimating the expression of Col I, ALP, OCN and OPN. Despite that, till date, there is no marker which could demonstrate for certain, the differentiation of human DPSCs and SHED towards the odontogenic and osteogenic lineage. This review suggests that SHED are noticeably different from DPSCs and exhibited higher capacity for osteogenic differentiation compared to DPSCs. On the other hand, different expression levels are shown by SHED and DPSCs with regards to the osteoblast markers for osteoblastic differentiation, where, SHED expressed higher levels of ALP, Col I and OCN compared to DPSCs.

Keywords: Dental pulp stem cells (DPSCs), differentiation, expression, odontogenic markers, osteoblast markers, stem cells from human exfoliated deciduous tooth (SHED).

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Article Details

VOLUME: 12
ISSUE: 1
Year: 2017
Page: [71 - 79]
Pages: 9
DOI: 10.2174/1574888X11666160815095733
Price: $58

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