Detection of Quinolone Resistance in Salmonella typhimurium Pig Isolates Determined by gyrA Gene Mutation Using PCR- and Sequence-Based Techniques within the gyrA Gene

Title:Detection of Quinolone Resistance in Salmonella typhimurium Pig Isolates Determined by gyrA Gene Mutation Using PCR- and Sequence-Based Techniques within the gyrA Gene

Volume: 22 Issue: 33

Author(s): Guadalupe Patricia Macías Farrera, Esvieta Tenorio Borroto, Fabiola Rivera Ramírez, Juan Carlos Vázquez Chagoyán, Martín Talavera Rojas, Gilberto Yong Angel and Roberto Montes de Oca Jimenez

Affiliation:

Keywords: Drug resistance, gyrase A, S. typhimurium, quinolones, bioinformatics.

Abstract: Background: The emergence of reduced susceptibility to fluoroquinolones among Salmonella enterica serotype Typhimurium isolates leading to clinical failure of treatment poses a great therapeutic challenge.

Methods: The current study is focused on the evaluation of the minimum inhibitory concentration (MIC) of quinolones in 29 Salmonella typhimurium of 86 Salmonella spp. strains, obtained from pigs from the State of Mexico. The MIC was performed with the Kirby-Bauer method. On the other hand, the GyrA gene was sequenced. The present study was undertaken to describe the resistance profiles and fluoroquinolone resistance mechanism of Salmonella Typhimurium.

Results: The DNA sequence of the gyrA genes from Salmonella enterica serovar typhimurium revealed strong similarity between gyrA and its counterpart in Escherichia coli. The sequencing of quinolone resistance-determining region (QRDR) of the gyrA gene showed the presence of mutation at either S83 or at D87 in almost all the Salmonella typhimurium isolates.

Conclusion: This mutation, although phenotypically expressed as decreased susceptibility to fluoroquinolones goes undetected by the disk diffusion method using the present method of Kirby-Bauer. Hence, it can increase morbidity and mortality due to delay in appropriate antibiotic treatment.

Cite this article as:

Macías Farrera Patricia Guadalupe, Borroto Tenorio Esvieta, Ramírez Rivera Fabiola, Vázquez Chagoyán Carlos Juan, Rojas Talavera Martín, Angel Yong Gilberto and de Oca Jimenez Montes Roberto, Detection of Quinolone Resistance in Salmonella typhimurium Pig Isolates Determined by gyrA Gene Mutation Using PCR- and Sequence-Based Techniques within the gyrA Gene, Current Pharmaceutical Design 2016; 22 (33) . https://dx.doi.org/10.2174/1381612822666160803165645