Title:Chemical Constituents from Hymenocallis littoralis
VOLUME: 13 ISSUE: 7
Author(s):Ning Chen, Yubin Ji, Weiguo Zhang, Ying Xu, Xinjia Yan, Yunfeng Sun, Hui Song, Changru Xu, Liping Cai, Hongxin Zheng and Zheng Xiang
Affiliation:Center of Research on Life Science and Environmental Science, Harbin University of Commerce, Harbin 150076, P.R. China., Genenal surgery, The first affiliated hospital of Dalian Medical University, Dalian 116000, P.R. China.
Keywords:Alkaloid, amaryllidaceae, cell line, cytotoxic activity, Hymenocallis littoralis, nuclear magnetic resonance.
Abstract:Background: Hymenocallis littoralis is the unique genus in China and
famous for its high content of alkaloids with anti-tumor activity. However, about
twenty alkaloids were isolated and identified in this plant.
Methods: The dichloromethane extract of H. littoralis (alkalization with 1% NaOH
for 24h) was further subjected to normal-phase silica, ODS silica gel column chromatography,
and semi-preparative HPLC chromatography, respectively. Chemical
structures of these compounds were identified and elucidated by spectroscopic
methods including 1D-NMR, 2D-NMR, and HR-MS. The cytotoxic activities of the
new component were determined with the cell lines of HepG-2, LoVo, Hela and
A549 using the MTT method after incubation for 48 h.
Results: One new alkaloid, named Hymenolitatine (1), with two known compounds was obtained from
the bulb of H. littoralis. The chemical structure of the new natural product was elucidated by spectroscopic
analysis and comparison with literature data. The two known compounds were identified as
trispheridine (2), tazettine (3), on the basis of comparison of their spectral data with the reported data,
respectively. The IC50 values of the compound 1 on HepG-2, LoVo, Hela and A549 cell lines were
75.19, 69.81, 96.37 and 102.53 μM, respectively.
Conclusion: This is the first report on the isolation and identification of Hymenolitatine (1), and this
novel compound exhibited moderate cytotoxic activities against HepG-2, LoVo, Hela and A549 cell
lines in vitro. Compound 2 was isolated from the title plant for the first time.
