Current Drug Delivery

Istvan Toth
School of Pharmacy,University of Queensland
Brisbane, 4072
Australia

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Highly Immunogenic Trimethyl Chitosan-based Delivery System for Intranasal Lipopeptide Vaccines against Group A Streptococcus

Author(s): Nirmal Marasini, Khairunnisa Abdul Ghaffar, Ashwini Kumar Giddam, Michael R. Batzloff, Michael F. Good, Mariusz Skwarczynski, Istvan Toth.

Graphical Abstract:


Abstract:

Background: Group A streptococcus (GAS) primarily colonizes the mucosal region of the upper respiratory tract, slowly leading to systemic infections. Thus, GAS-specific antibody responses are desirable at mucosal sites for early prevention against GAS colonization.

Methods: Herein, we developed a potent nanoliposomes-based delivery system for mucosally active lipid core peptide (LCP)-based vaccines.

Results: Trimethyl chitosan (TMC)-coated liposomes that bore a B-cell epitope derived from GAS Mprotein, stimulated potent epitope-specific mucosal and systemic antibody titres after only one boost following intranasal immunization in Swiss outbred mice. The immune responses were durable even at day 139 post-primary immunization.

Conclusion: The enhanced vaccine efficacy, lowered dose, and simple and cost-effective process of producing the coated nanoliposomes should be particularly useful in developing potent peptide-based vaccines to prevent infections at the mucosal sites.

Keywords: Chitosan, group A streptococcus, lipopeptides, liposomes, nasal delivery, peptide vaccines.

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Article Details

VOLUME: 14
ISSUE: 5
Year: 2017
Page: [701 - 708]
Pages: 8
DOI: 10.2174/1567201813666160721141322
Price: $58