Highly Immunogenic Trimethyl Chitosan-based Delivery System for Intranasal Lipopeptide Vaccines against Group A Streptococcus
Khairunnisa Abdul Ghaffar,
Ashwini Kumar Giddam,
Michael R. Batzloff,
Michael F. Good,
Background: Group A streptococcus (GAS) primarily colonizes the mucosal region of the
upper respiratory tract, slowly leading to systemic infections. Thus, GAS-specific antibody responses
are desirable at mucosal sites for early prevention against GAS colonization.
Methods: Herein, we developed a potent nanoliposomes-based delivery system for mucosally active
lipid core peptide (LCP)-based vaccines.
Results: Trimethyl chitosan (TMC)-coated liposomes that bore a B-cell epitope derived from GAS Mprotein,
stimulated potent epitope-specific mucosal and systemic antibody titres after only one boost
following intranasal immunization in Swiss outbred mice. The immune responses were durable even at
day 139 post-primary immunization.
Conclusion: The enhanced vaccine efficacy, lowered dose, and simple and cost-effective process of
producing the coated nanoliposomes should be particularly useful in developing potent peptide-based
vaccines to prevent infections at the mucosal sites.
Keywords: Chitosan, group A streptococcus, lipopeptides, liposomes, nasal delivery, peptide vaccines.
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