Separation of Metal–Trifluoperazine Hydrochloride Complexes Using Zwitterionic Ion Chromatography (ZIC) Coupled Online with ICP-AES

Author(s): Andreas Seubert, Ashraf Saad Rasheed*.

Journal Name: Current Pharmaceutical Analysis

Volume 13 , Issue 4 , 2017

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Abstract:

Background: Despite the numerous studies about ZIC and ZIC-HILIC retention modes, the mechanism has not been fully investigated and there are no detailed mechanistic concepts in the literature. Some of the pharmaceutical compounds can act as a strong chelator for multiple charged metal ions of biological importance. Trifluoperazine hydrochloride (TFPH) is a strong chelator for multiple charged metal ions of biological importance (Pd2+, Pt4+, V5+) and other metals. It will be interesting to see if the metal–complexes are also separated using the zwitterionic stationary phase.

Methods and Result: The study of the influence of pH and eluent concentration of metal-TFPH complexes selectivity should give a clue about the properties of the individual zwitterionic stationary phases and thus about the separation mechanism. The retention factors of Pt(IV)-TFPH, Pd(II)-TFPH and V(V)-TFPH complexes decreased with an increasing buffer concentration. The ZIC5 stationary phase as well as the examined commercial stationary phases ZIC-HILIC and ZIC-pHILIC exhibit the same behavior. The retention of the TFPH-Pt(IV), TFPH-Pd(II) and TFPH-V(V) complexes with their positive charge decreased on ZIC5, ZIC-HILIC and ZIC-pHILIC columns when the pH increased in the range between 3 to 6. The results of variations of buffer concentration and mobile phase pH show that metal- TFPH complexes can be separated based cation-exchange interactions and electrostatic interaction between the stationary phase and analyte.

Conclusion: The current study confirmed the ability of ZIC-HILIC stationary phases to separate metalcomplexes. It could be demonstrated that metal-TFPH separations on zwitterionic stationary phase are mainly driven by a cation exchange mechanism.

Keywords: Trifluoperazine, ion exchange, zwitterionicstationary phase, palladium (II), platinum (IV), vanadium (V).

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Article Details

VOLUME: 13
ISSUE: 4
Year: 2017
Page: [328 - 333]
Pages: 6
DOI: 10.2174/1573412912666160720114147
Price: $58

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