Current Biochemical Engineering

Ruey-Shin Juang
Department of Chemical and
Materials Engineering
Chang Gung University
Kwei-Shan, Taoyuan
Taiwan

T.C. Ling
University of Malaya
Kuala Lumpur
Malaysia

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Genetic Manipulation to Increase Lipase Production in Microorganisms – A Recent Review

Author(s): Chung Hong Tan, Pau Loke Show, Tau Chuan Ling, Hon Loong Lam.

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Abstract:

Enzymes are important biocatalysts that exist naturally in all living organisms. Nowadays, enzymes have been widely applied to modern industries because biological catalysts are substratespecific, highly active and operate within mild environments. However, the cost of culturing proteinrich microorganisms as well as extracting and purifying the enzymes are high. One solution is to genetically modify the microorganisms for high protein expression. Therefore, this review discusses the mechanism of various mutagenesis techniques (physical and chemical) as well as the potential genetic modification to increase protein yields in microorganisms.

Keywords: Enzymes, genetic engineering, micro-organisms, protein expression, biocatalysts.

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Article Details

VOLUME: 4
ISSUE: 1
Year: 2017
Page: [34 - 42]
Pages: 9
DOI: 10.2174/2212711903666160712223634
Price: $58