Objectives: This study investigated the neuroprotective effects of Jatrorrhizine in rat cortical
Method: The effects of Jatrorrhizine on hydrogen peroxide (H2
)-induced cell lesion, levels of lipid
peroxidation and antioxidant enzyme activities were investigated in rat cortical neurons. Levels of mitochondrial
membrane potential (MMP) and intracellular reactive oxygen species (ROS) were measured by
fluorescent rhodamine staining and 2’,7’-dichlorfluorescein-diacetate staining, respectively. ATP content
was measured by a high performance liquid chromatography. The protein levels for Bax, Bcl2 and
cleaved caspase-3 were analyzed by western blot protein expression.
Results: There was a significant reduction in cell viability and activities of Superoxide dismutase and
glutathione peroxidase for the cortical neurons after exposure to 50μM H2
for 12h. The hydrogen
peroxide increased the production of malondialdehyde and ROS but decreased MMP and ATP in the
neurons. However, pretreatment with different concentrations of Jatrorrhizine (5-20μM) inhibited
-induced neurotoxicity markedly. Jatrorrhizine also attenuated the H2
-induced Bcl-2/Bax ratio
reduction and caspase-3 activation in these neurons.
Conclusions: Our findings suggest that Jatrorrhizine plays a critical neuroprotective role in H2
induced apoptosis through its anti-oxidative actions. This may allow Jatrorrhizine to be a novel therapeutic
with its high bioavailability to treat Alzheimer’s disease.