Monitoring of multiple sclerosis (MS) requires additional molecular
markers. Recently, we used original TCA-precipitation/extraction approach in
combination with MALDI TOF/TOF mass-spectrometry and identified earlier
unknown 48 kDa form of the unconventional myosin IC isoform b (Myo1C) in
blood serum of the MS patients. Further examination of TCA-extracted fraction of
blood serum of these patients by means of thin-layer chromatography and HPLC
gel-filtration allowed detecting 300-500 Da peptides. MALDI TOF/TOF massspectrometry
of these peptides showed that they contain Ser-Pro-Cys amino acid
sequence. We discussed potential mechanisms of a release of these peptides that
were earlier unknown in blood serum of the MS patients.
Keywords: Multiple sclerosis, blood serum, peptides.
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