Identification of SER-PRO-CYS Peptide in Blood Serum of Multiple Sclerosis Patients

Author(s): Severyn Myronovkij, Nazar Negrych, Tetyana Nehrych, Volodymyr Tkachenko, Serhiy Souchelnytskyi, Rostyslav Stoika, Yuriy Kit.

Journal Name: Protein & Peptide Letters

Volume 23 , Issue 9 , 2016

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Abstract:

Monitoring of multiple sclerosis (MS) requires additional molecular markers. Recently, we used original TCA-precipitation/extraction approach in combination with MALDI TOF/TOF mass-spectrometry and identified earlier unknown 48 kDa form of the unconventional myosin IC isoform b (Myo1C) in blood serum of the MS patients. Further examination of TCA-extracted fraction of blood serum of these patients by means of thin-layer chromatography and HPLC gel-filtration allowed detecting 300-500 Da peptides. MALDI TOF/TOF massspectrometry of these peptides showed that they contain Ser-Pro-Cys amino acid sequence. We discussed potential mechanisms of a release of these peptides that were earlier unknown in blood serum of the MS patients.

Keywords: Multiple sclerosis, blood serum, peptides.

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Article Details

VOLUME: 23
ISSUE: 9
Year: 2016
Page: [808 - 811]
Pages: 4
DOI: 10.2174/0929866523666160622215628
Price: $58

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