A Facile and Microwave-assisted Rapid Synthesis of 2-Arylamino-4-(3′-indolyl)- thiazoles as Apoptosis Inducing Cytotoxic Agents

Author(s): Mukund P. Tantak , Dipanwita Das Mukherjee , Anil Kumar , Gopal Chakrabarti , Dalip Kumar .

Journal Name: Anti-Cancer Agents in Medicinal Chemistry

Volume 17 , Issue 3 , 2017

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Abstract:

Background and Objective: The clinical success of the chemotherapeutic drugs is restricted by the nonspecific toxicity-related adverse side effects. The diverse implication of indoles and thiazoles in medicinal chemistry prompted us to develop a new series of novel 2-aryl-amino-4-(3′-indolyl)thiazoles as more effective and less toxic anti-cancer compounds.

Method and Results: One-pot microwave-assisted rapid and high yielding synthesis of 2-arylamino-4-(3′- indolyl)thiazoles involved the reaction of easily available α-tosyloxy-ketones with N-arylthioureas in polyethylene glycol-400 (PEG-400). In vitro cytotoxicity study of 2-arylamino-4-(3′-indolyl)thiazoles against a panel of human cancer cell lines by MTT assay revealed IC50 values in the low micromolar range. Of the fifteen synthesized arylaminothiazoles, compounds 17b, 17d, 17g and 17il showed significant anti-proliferative activity against the selected cancer cell lines with IC50 < 10 μM. The compound 17b was identified as the most potent ligand of the series, which exhibited good cytotoxic activity against MCF-7 breast cancer cells with an IC50 value of 1.86 μM but minimal toxicity on normal human cells. Investigation of the underlying mechanism by flow cytometry indicated that 17b induced ROS-mediated apoptosis in MCF-7 cells in a dose-dependent manner as supported by upregulation of Bax and caspase-3 and down-regulation of Bcl-2 (by Western blot).

Conclusion: Developed an efficient and eco-friendly synthesis for 2-arylamino-4-(3′-indolyl)thiazoles, and their in vitro cytotoxicity studies demonstrate that compound 17b exhibits significant anti-proliferative activity against MCF-7 (breast cancer) cells by activating ROS-mediated apoptosis through the mitochondrial apoptosis pathway.

Keywords: Indoles, arylaminothiazoles, microwave irradiation, one-pot synthesis, cytotoxicity, apoptosis.

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Article Details

VOLUME: 17
ISSUE: 3
Year: 2017
Page: [442 - 455]
Pages: 14
DOI: 10.2174/1871520616666160622095406
Price: $58

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