Cell-free translation systems facilitate rapid production of specific proteins
and are particularly suited as high-throughput methods for whole-genome
protein synthesis. Moreover, these systems do not rely on living cells, thereby
allowing the synthesis of unstable or cytotoxic proteins in vitro. In this review, we
describe the principles and potential applications of cell-free protein translation
systems and the future prospects of proteomics approaches using next-generation
sequencing and cell-free expression technologies.
Keywords: Cell-free, IVV (in vitro virus), mRNA display, PPI (protein-protein interaction), Proteomics, PURE system.
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