Introduction: Aristolochic acid I, a potent human nephrotoxic and carcinogenic compound
produced by aristolochia plants, it is responsible for Balkan endemic nephropathy and is associated
with urothelial carcinoma.
Method and Materials: A new method named air-assisted liquid-liquid microextraction has been developed
for enrichment of analyte. The mixture of organic extractant and aqueous solution containing
the analyte was sucked into a 5-mL glass syringe and pushed into the tube for times. This action produced
a cloudy solution which indicated dispersion of organic solvent into aqueous solution, thereupon
extraction procedure. The chromatographic separation was performed on a C18 column and mobile
phase consist of 15% methanol, 20% acetonitrile, and 65% aqueous acetate buffer solution (pH=5.3).
Flow rate of 1 mL min-1 and wavelength of 254 nm were used in analysis.
Results: The limits of detection and quantification were obtained as 0.16 and 0.53 µg L-1, respectively.
Intra- and inter-day precisions were calculated as relative standard deviations and obtained as 0.66%
and 0.72% for 10 µg L-1, respectively.
Keywords: Aristolochic acid, flour, fruit, high–performance liquid chromatography, microextraction, urine.
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