Background: Solifenacin is used for the treatment of overactive bladder. At present, a number
of separation and determination techniques, including high-performance liquid chromatography, Liquid
chromatography–tandem mass spectrometry and spectrophotometric techniques have been used for the
analysis of solifenacin in different samples.
Objective: In the present work, for the first time, a Hollow fiber based liquid phase microextraction (HFLPME)
followed by high performance liquid chromatography (HPLC-UV) was used for extraction of
solifenacin from biological samples.
Method: Chemometric method was used for optimization of the effects of different parameters influencing
the extraction efficiency of the proposed method. The organic liquid membrane consists of 1-Octanol
immobilized in the pores of a hollow fiber wall. A pH gradient was applied to migrate analytes from alkaline
sample solution with pH 10.9, through the organic liquid membrane into an acidic acceptor solution
with pH 2.9. which was located inside the lumen of hollow fiber.
Results: Under optimized experimental conditions, extraction recoveries higher than 85% were obtained
in different biological matrices which resulted in preconcentration factors higher than 163 and acceptable
repeatability(RSD% within day:3.47%..RSD% between day: 4.12%).
Conclusion: The method offers good linearity with estimation of coefficient higher than 0.9900. Finally,
it was applied to the detection and determination of solifenacin in human plasma and urine samples.