Background: Antiviral response rates are highly variable in Hepatitis B chronically infected
individuals due to its extensive genetic variability. This genetic inconsistency varies from region to
region and highly affects the severity of disease, its progression and treatment outcome. Idea of the
existing work is to come across out antiviral response rates of interferon and lamivudine in HBV
positive individuals after one year of treatment and their correlation with genotypes in Pakistani
Methods: Sera samples were collected from treated patients and then were subjected to DNA extraction
followed by genotyping. Quantification analysis using real time PCR was performed before the
treatment, after six months interval and finally in the follow up period of 6 months.
Results: Out of total 100 (75% males and 25% females) selected 74 patients [(52/74 (70%) male and
22/74 (30%) females] completed the lamivudine standard therapy (100mg/day) with an end treatment
response (ETR) rate of 43/74 (58.1%) while 26 patients were getting interferon-alpha (IFN-α) treatment
(5mIU/inj.) showed 4/26 (15.38%) ETR and 12/26 (46.1%), whereas 10/26 (38.4%) were nonresponders.
In interferon treated patients, ETR was higher in genotype A, B and C in number of patients
respectively 1, 6 and 8. Whereas it was more in genotype D, A, B and F 14, 13, 2, (61.5%, 56.5%, 50%
and 25% respectively) after lamivudine treatment.
Conclusion: Overall, the treatment response was significantly higher (p-value < 0.05) in patients under
40 years of age. The current study shows a high ETR to antiviral therapy in Pakistan in comparison to
other parts of the globe for a defined period of one year.