Oxidative damage to the constituents of the eye lens is a major mechanism in the initiation
and development of cataract. Lunasin, a 43-amino acids chemoprevention peptide, has been proved to
possess potent anti-oxidative activity other than its established anticancer activities. Herein, we explored
whether lunasin has preventative effects on d-galactose-induced experimental cataract in rat.
After modeling, SD rats were administrated by instillation, 80 M of lunasin eye drops to each eye
thrice daily and consecutively for 30 days. As a result, lunasin treatment effectively inhibited the progression
of d-galactose-induced experimental cataract, and protected the lenses of rats from oxidative
damage and attenuated the lipid peroxidation through up-regulation of antioxidant enzymes, and inhibited the activation of
polyol pathway by decreasing AR activity. Additionally, in vitro studies proved that lunasin treatment could protect human
lens epithelial cells (hLECs) against d-galactose induced cell damage and apoptosis, and up-regulate antioxidant enzymes.
This is the first demonstration that lunasin could inhibit d-galactose-induced experimental cataract in rats by protecting
against oxidative damage and inhibiting the activation of polyol pathway.
Keywords: Antioxidant enzymes, cataract, chemoprevention peptide, lunasin, oxidative damage, polyol pathway.
Rights & PermissionsPrintExport